Antigen

Antigen Class

TFs and others

Antigen

MYCN

Cell type

Cell type Class

Neural

Cell type

IMR-32

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

source_name

MYCN_ChIPseq

cell line

IMR32 cells

cell type

Neuroblastoma

treatment

siCtrl

chip antibody

MYCN (Active motif, 61185)

geo_loc_name

missing

collection_date

missing

Sequenced DNA Library

library_name

GSM6346069

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody following the Active Motif's instructions of the ChIP-IT High Sensitivity Kit. Libraries were prepared according to Illumina's TruSeq ChIP Sample Preparation Guide.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

34010674

Reads aligned (%)

91.0

Duplicates removed (%)

15.5

Number of peaks

22631 (qval < 1E-05)

hg19

Number of total reads

34010674

Reads aligned (%)

90.5

Duplicates removed (%)

16.7

Number of peaks

22513 (qval < 1E-05)