Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYCN

Cell type

Cell type Class
Neural
Cell type
IMR-32
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
MYCN_ChIPseq
cell line
IMR32 cells
cell type
Neuroblastoma
treatment
siCtrl
chip antibody
MYCN (Active motif, 61185)
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM6346069
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody following the Active Motif's instructions of the ChIP-IT High Sensitivity Kit. Libraries were prepared according to Illumina's TruSeq ChIP Sample Preparation Guide.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
34010674
Reads aligned (%)
91.0
Duplicates removed (%)
15.5
Number of peaks
22631 (qval < 1E-05)

hg19

Number of total reads
34010674
Reads aligned (%)
90.5
Duplicates removed (%)
16.7
Number of peaks
22513 (qval < 1E-05)

Base call quality data from DBCLS SRA